{"id":9350,"date":"2011-07-18T13:53:57","date_gmt":"2011-07-18T13:53:57","guid":{"rendered":"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-analytickej-cytometrie\/"},"modified":"2022-11-05T18:45:12","modified_gmt":"2022-11-05T18:45:12","slug":"laboratorium-analytickej-cytometrie","status":"publish","type":"page","link":"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-analytickej-cytometrie\/","title":{"rendered":"Laborat\u00f3rium analytickej cytometrie"},"content":{"rendered":"<p style=\"text-align: justify\">Laborat\u00f3rium analytickej cytometrie Pr\u00edrodovedeckej fakulty UPJ\u0160 (LAC) pod veden\u00edm <a href=\"mailto:peter.fedorocko@upjs.sk\">prof. RNDr. Petra Fedoro\u010dka, <\/a><a>CSc.<\/a> je vybaven\u00e9 \u0161pi\u010dkov\u00fdm konfok\u00e1lnym mikroskopom TCS SP5 X, ktor\u00fd pl\u00e1novite doplnil u\u017e existuj\u00facu infra\u0161trukt\u00faru tohto laborat\u00f3ria pozost\u00e1vaj\u00facu z invertovan\u00e9ho fluorescen\u010dn\u00e9ho mikroskopu DMI 6000 B vybaven\u00e9ho mikromanipul\u00e1torom a&nbsp;kultiva\u010dnou kom\u00f4rkou pre zber d\u00e1t zo \u017eiv\u00fdch buniek. Obidva mikroskopick\u00e9 syst\u00e9my dodala spolo\u010dnos\u0165 Leica Microsystems.<\/p>\n<p style=\"text-align: justify\">\u0160pi\u010dkov\u00e1 mikroskopick\u00e1 technika v celkovej hodnote viac ako 658 tis. \u20ac bola zak\u00fapen\u00e1 z prostriedkov projektu APVV VVCE-0001-07 \u2013 \u201eCentrum pre v\u00fdskum signal\u00f3mu\u201c a z projektu&nbsp; \u0160F \u201eSEPO I &#8211;&nbsp; Sie\u0165 excelentn\u00fdch pracov\u00edsk pre onkol\u00f3giu\u201c (ITMS 26220120024).<\/p>\n<p style=\"text-align: justify\"><strong>Konfok\u00e1lny mikroskop Leica TCS SP5 X<\/strong> je zariadenie ur\u010den\u00e9 na trojrozmern\u00fa mikroskopick\u00fa anal\u00fdzu a sledovanie dynamick\u00fdch zmien v&nbsp;\u010dase v&nbsp;bunk\u00e1ch a&nbsp;tkaniv\u00e1ch<br \/>\ns mo\u017enos\u0165ou fluorescen\u010dnej spektr\u00e1lnej anal\u00fdzy. Okrem toho umo\u017e\u0148uje aj kolokaliza\u010dn\u00e9 \u0161t\u00fadie na subcelul\u00e1rnej \u00farovni. Ke\u010f\u017ee detekcia je zalo\u017een\u00e1 na princ\u00edpe fluorescencie, anal\u00fdza je limitovan\u00e1 na \u0161trukt\u00fary s&nbsp;autofluorescenciou alebo zna\u010den\u00e9 fluorescen\u010dne pomocou r\u00f4znych farb\u00edv. Medzi \u0161tandardn\u00e9 aplik\u00e1cie patr\u00ed subcelul\u00e1rna lokaliz\u00e1cia r\u00f4znych prote\u00ednov, \u0161trukt\u00farne zmeny bunkov\u00fdch organel, zmeny fyziologick\u00fdch parametrov bunky ako s\u00fa pH, mitochondri\u00e1lny potenci\u00e1l, hladina Ca<sup>2+<\/sup> a pod. Z\u00e1rove\u0148 je mo\u017en\u00e9 vykon\u00e1va\u0165 aj sofistikovanej\u0161ie anal\u00fdzy vz\u00e1jomnej kolokaliz\u00e1cie \u0161trukt\u00far pomocou met\u00f3dy FRET (Fluorescence Resonance Energy Transfer).<\/p>\n<p style=\"text-align: justify\">Intuit\u00edvne a jednoduch\u00e9 softv\u00e9rov\u00e9 rozhranie a plne automatizovan\u00fd syst\u00e9m umo\u017e\u0148uje \u013eahk\u00e9 zacvi\u010denie a obsluhu. Otv\u00e1raj\u00fa sa tak atrakt\u00edvne mo\u017enosti vyu\u017eitia t\u00fdchto syst\u00e9mov aj vo v\u00fdu\u010dbe \u0161tudentov a&nbsp;v ich zasv\u00e4covan\u00ed do sveta molekul\u00e1rnej cytol\u00f3gie.<\/p>\n<p style=\"text-align: justify\">V&nbsp;z\u00e1vere roka 2010 bolo toto laborat\u00f3rium vybaven\u00e9 \u010fal\u0161\u00edm \u0161pi\u010dkov\u00fdm pr\u00edstrojom \u2013 <strong>prietokov\u00fdm cytometrom CyFlow ML a prietokov\u00fdm cytometrom so sortrom BD FACSAria<\/strong> &#8211; z&nbsp;projektu \u201eBudovanie infra\u0161trukt\u00fary v centre excelentnosti SEPO-II\u201c (ITMS 26220120039)&nbsp; vr\u00e1tane obnovy \u010fal\u0161ej laborat\u00f3rnej techniky v&nbsp;celkovej hodnote skoro 750 tis. \u20ac.<\/p>\n<p style=\"text-align: justify\">Prietokov\u00fd cytometer\/sorter&nbsp; BD FACSAria je zariadenie umo\u017e\u0148uj\u00face multiparametrick\u00fa anal\u00fdzu fluorescencie r\u00f4znych \u010dast\u00edc (naj\u010dastej\u0161ie buniek) pripraven\u00fdch v suspenzii a ich n\u00e1sledn\u00e9 delenie na samostatn\u00e9 popul\u00e1cie na z\u00e1klade \u013eubovo\u013enej kombin\u00e1cie analyzovan\u00fdch parametrov. BD FACSAria umo\u017e\u0148uje analyzova\u0165 a triedi\u0165 r\u00fdchlos\u0165ou a\u017e 70 000 \u010dast\u00edc za sekundu. Takto je mo\u017en\u00e9 analyzova\u0165 a izolova\u0165 aj vz\u00e1cne sa vyskytuj\u00face elementy ako napr. kme\u0148ov\u00e9 bunky, n\u00e1dorov\u00e9 a&nbsp;nen\u00e1dorov\u00e9 bunky a pod.<\/p>\n<p style=\"text-align: justify\">Laborat\u00f3rium analytickej cytometrie je lokalizovan\u00e9 jednak v&nbsp;b\u00fdval\u00fdch zdevastovan\u00fdch skladov\u00fdch priestoroch \u00dastavu biologick\u00fdch a&nbsp;ekologick\u00fdch vied PF UPJ\u0160 v&nbsp;RBL pavil\u00f3ne a&nbsp;jednak v&nbsp;rekon\u0161truovan\u00fdch laborat\u00f3rnych priestoroch \u00fastavu na M\u00e1nesovej 23. Rekon\u0161trukcia a&nbsp;prestavba na laborat\u00f3rne priestory bola financovan\u00e1 z&nbsp;prostriedkov uveden\u00fdch projektov v&nbsp;celkovej hodnote viac ako 97 tis. \u20ac.<\/p>\n<p style=\"text-align: justify\">\n<p style=\"text-align: center\"><img decoding=\"async\" alt=\"\" border=\"0\" src=\"http:\/\/www.upjs.sk\/\/app\/uploads\/2022\/09\/loga-eu-spolu-4.jpg\" style=\"height:113px;width:490px\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Laborat\u00f3rium analytickej cytometrie Pr\u00edrodovedeckej fakulty UPJ\u0160 (LAC) pod veden\u00edm prof. RNDr. Petra Fedoro\u010dka, CSc. je vybaven\u00e9 \u0161pi\u010dkov\u00fdm konfok\u00e1lnym mikroskopom TCS SP5 X, ktor\u00fd pl\u00e1novite doplnil u\u017e existuj\u00facu infra\u0161trukt\u00faru tohto laborat\u00f3ria pozost\u00e1vaj\u00facu z invertovan\u00e9ho fluorescen\u010dn\u00e9ho mikroskopu DMI 6000 B vybaven\u00e9ho mikromanipul\u00e1torom a&nbsp;kultiva\u010dnou kom\u00f4rkou pre zber d\u00e1t zo \u017eiv\u00fdch buniek. Obidva mikroskopick\u00e9 syst\u00e9my dodala spolo\u010dnos\u0165 Leica Microsystems. &hellip; <a href=\"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-analytickej-cytometrie\/\">\u010c\u00edta\u0165 \u010falej<\/a><\/p>\n","protected":false},"author":1,"featured_media":9353,"parent":3973,"menu_order":8,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_editorskit_title_hidden":false,"_editorskit_reading_time":0,"_editorskit_is_block_options_detached":false,"_editorskit_block_options_position":"{}","footnotes":""},"class_list":["post-9350","page","type-page","status-publish","has-post-thumbnail","hentry"],"acf":{"acf_link_na_externy_obsah":""},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.8 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Laborat\u00f3rium analytickej cytometrie - UPJS Kosice<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-analytickej-cytometrie\/\" \/>\n<meta property=\"og:locale\" content=\"sk_SK\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Laborat\u00f3rium analytickej cytometrie - UPJS Kosice\" \/>\n<meta property=\"og:description\" content=\"Laborat\u00f3rium analytickej cytometrie Pr\u00edrodovedeckej fakulty UPJ\u0160 (LAC) pod veden\u00edm prof. 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