{"id":13900,"date":"2023-04-05T11:24:53","date_gmt":"2023-04-05T11:24:53","guid":{"rendered":"https:\/\/www.upjs.sk\/testovacia\/?page_id=13900"},"modified":"2023-04-13T09:10:38","modified_gmt":"2023-04-13T09:10:38","slug":"test-kotva","status":"publish","type":"page","link":"https:\/\/www.upjs.sk\/testovacia\/en\/test-kotva\/","title":{"rendered":"test kotva"},"content":{"rendered":"\n
    \n
  1. V\u00fdvoj efekt\u00edvnych geneticky k\u00f3dovan\u00fdch fotosenzibiliz\u00e1torov<\/a><\/li>\n\n\n\n
  2. kotvvvvvvvaaaaaaaaa<\/a><\/li>\n\n\n\n
  3. kotva1<\/a><\/li>\n\n\n\n
  4. kotva4<\/a><\/li>\n<\/ol>\n\n\n\n

    Hlavn\u00fdm cie\u013eom tohto <\/a>projektu je vyvin\u00fa\u0165 molekul\u00e1rnu met\u00f3du, pomocou ktorej budeme schopn\u00ed transformova\u0165 r\u00f4zne flavoprote\u00edny na \u00fa\u010dinn\u00e9 fotosenzibiliz\u00e1tory produkuj\u00face singletov\u00fd kysl\u00edk s vysok\u00fdm kvantov\u00fdm v\u00fd\u0165a\u017ekom. Vybran\u00e9 flavoprote\u00edny a vhodne navrhnut\u00e9 mutanty bud\u00fa exprimovan\u00e9 v Escherichia coli <\/em>a purifikovan\u00e9 be\u017en\u00fdmi met\u00f3dami. Vytvoren\u00e9 prote\u00edny konjugujeme so \u0161pecifick\u00fdmi DARPin-mi (skupina navrhnut\u00fdch ankyr\u00ednov\u00fdch repetit\u00edvnych prote\u00ednov \u2013 prote\u00ednov\u00e1 skupina, ktor\u00e1 predstavuje anal\u00f3gy protil\u00e1tok), ktor\u00e9 \u0161pecificky rozpozn\u00e1vaj\u00fa receptory HER2. Tieto konjug\u00e1ty sa pou\u017eij\u00fa na bunkovej \u00farovni na demon\u0161tr\u00e1ciu ich \u00fa\u010dinnosti, tj. selekt\u00edvnej v\u00e4zby na bunky s receptormi HER2 a toxicity pripojen\u00fdch flavoprote\u00ednov vyvolanej svetlom. Pri dosiahnut\u00ed tohto cie\u013ea z\u00edskame n\u00e1stroj na vytvorenie generickej met\u00f3dy na transform\u00e1ciu v z\u00e1sade ak\u00fdchko\u013evek flavoprote\u00ednov na \u00fa\u010dinn\u00e9 fotosenzibiliz\u00e1tory.<\/p>\n\n\n\n

    Prij\u00edmaj\u00faci profesor:<\/strong> prof. RNDr. Pavol Mi\u0161kovsk\u00fd, DrSc., E-mail: pavol.miskovsky@upjs.sk<\/a><\/p>\n\n\n\n

    Zodpovedn\u00fd pracovn\u00edk:<\/strong> doc. RNDr. Erik Sedl\u00e1k, DrSc., E-mail: erik.sedlak@upjs.sk<\/a><\/p>\n\n\n\n

    Odborn\u00e9 po\u017eiadavky\/krit\u00e9ri\u00e1:<\/strong><\/p>\n\n\n\n

    \n\n\n\n

    Technologick\u00fd a inova\u010dn\u00fd park<\/strong><\/p>\n\n\n\n

    1. – kotva<\/a>. N\u00e1zov t\u00e9my: V\u00fdvoj efekt\u00edvnych geneticky k\u00f3dovan\u00fdch fotosenzibiliz\u00e1torov<\/strong><\/p>\n\n\n\n

    Anot\u00e1cia:<\/em><\/strong><\/p>\n\n\n\n

    Hlavn\u00fdm cie\u013eom tohto projektu je vyvin\u00fa\u0165 molekul\u00e1rnu met\u00f3du, pomocou ktorej budeme schopn\u00ed transformova\u0165 r\u00f4zne flavoprote\u00edny na \u00fa\u010dinn\u00e9 fotosenzibiliz\u00e1tory produkuj\u00face singletov\u00fd kysl\u00edk s vysok\u00fdm kvantov\u00fdm v\u00fd\u0165a\u017ekom. Vybran\u00e9 flavoprote\u00edny a vhodne navrhnut\u00e9 mutanty bud\u00fa exprimovan\u00e9 v Escherichia coli <\/em>a purifikovan\u00e9 be\u017en\u00fdmi met\u00f3dami. Vytvoren\u00e9 prote\u00edny konjugujeme so \u0161pecifick\u00fdmi DARPin-mi (skupina navrhnut\u00fdch ankyr\u00ednov\u00fdch repetit\u00edvnych prote\u00ednov \u2013 prote\u00ednov\u00e1 skupina, ktor\u00e1 predstavuje anal\u00f3gy protil\u00e1tok), ktor\u00e9 \u0161pecificky rozpozn\u00e1vaj\u00fa receptory HER2. Tieto konjug\u00e1ty sa pou\u017eij\u00fa na bunkovej \u00farovni na demon\u0161tr\u00e1ciu ich \u00fa\u010dinnosti, tj. selekt\u00edvnej v\u00e4zby na bunky s receptormi HER2 a toxicity pripojen\u00fdch flavoprote\u00ednov vyvolanej svetlom. Pri dosiahnut\u00ed tohto cie\u013ea z\u00edskame n\u00e1stroj na vytvorenie generickej met\u00f3dy na transform\u00e1ciu v z\u00e1sade ak\u00fdchko\u013evek flavoprote\u00ednov na \u00fa\u010dinn\u00e9 fotosenzibiliz\u00e1tory.<\/strong><\/p>\n\n\n\n

    Prij\u00edmaj\u00faci profesor:<\/strong> prof. RNDr. Pavol Mi\u0161kovsk\u00fd, DrSc., E-mail: pavol.miskovsky@upjs.sk<\/a><\/p>\n\n\n\n

    Zodpovedn\u00fd pracovn\u00edk:<\/strong> doc. RNDr. Erik Sedl\u00e1k, DrSc., E-mail: erik.sedlak@upjs.sk<\/a><\/p>\n\n\n\n

    Odborn\u00e9 po\u017eiadavky\/krit\u00e9ri\u00e1:<\/strong><\/p>\n\n\n\n

    Zisk titulu PhD. v \u0161tudijnom programe Biofyzika (resp. pr\u00edbuznom \u0161tudijnom programe, napr. Bioch\u00e9mia, Molekulov\u00e1 biol\u00f3gia), minim\u00e1lne tri publik\u00e1cie publikovan\u00e9 vo vedeck\u00fdch \u010dasopisoch veden\u00fdch v datab\u00e1ze WoS, minim\u00e1lne na jednej z nich prv\u00fd alebo kore\u0161ponduj\u00faci autor, sk\u00fasenosti s expresiou a purifik\u00e1ciou prote\u00ednov, ich charakteriz\u00e1ciou spektr\u00e1lnymi a termodynamick\u00fdmi met\u00f3dami.<\/p>\n\n\n\n

    2.-kotva1<\/a> N\u00e1zov t\u00e9my: V\u00fdvoj novej gener\u00e1cie cielen\u00fdch transportn\u00fdch syst\u00e9mov schopn\u00fdch viaza\u0165 \u0161irok\u00e9 spektrum lie\u010div a vytv\u00e1ra\u0165 vysoko afinitn\u00fa v\u00e4zbu so \u0161pecifick\u00fdmi receptormi na n\u00e1dorov\u00fdch bunk\u00e1ch<\/strong><\/p>\n\n\n\n

    Anot\u00e1cia:<\/strong><\/p>\n\n\n\n

    Vedeck\u00e9 \u00falohy v r\u00e1mci tejto t\u00e9my s\u00fa zalo\u017een\u00e9 na s\u00fa\u010dasn\u00fdch poznatkoch v oblasti v\u00fdvoja transportn\u00fdch syst\u00e9mov pre cielen\u00fd transport lie\u010div. Navrhovan\u00fd transportn\u00fd syst\u00e9m lie\u010div (DARLINK) je zalo\u017een\u00fd na rekombinantnej inov\u00e1cii pozost\u00e1vaj\u00facej zo spojenia DARPinov \u2013 maj\u00facich vysok\u00fa afinitu pre v\u00e4zbu na \u0161pecifick\u00e9 receptory n\u00e1dorov\u00fdch buniek \u2013 a endog\u00e9nnych lipoprote\u00ednov\u00fdch nano\u010dast\u00edc, ktor\u00e9 maj\u00fa zna\u010dn\u00fa kapacitu pre naviazanie hydrof\u00f3bnych resp. amfifiln\u00fdch lie\u010div. Konkr\u00e9tne, navrhovan\u00fd transportn\u00fd syst\u00e9m bude skon\u0161truovan\u00fd f\u00faziou molek\u00fal LDL (alebo HDL) so \u0161pecifick\u00fdmi DARPinmi, ktor\u00e9 maj\u00fa vysok\u00fa afinitu pre naviazanie na HER2 receptory nach\u00e1dzaj\u00face sa na povrchu n\u00e1dorov\u00fdch buniek mlie\u010dnej \u017e\u013eazy a taktie\u017e DARPinmi vyselektovan\u00fdmi pre v\u00e4zbu na prirodzen\u00fa a mutovan\u00fa formu EGFR, ktor\u00e9 s\u00fa lokalizovan\u00e9 na povrchu buniek p\u013e\u00facnych n\u00e1dorov. Bunky n\u00e1doru mlie\u010dnej \u017e\u013eazy boli vybran\u00e9 ako modelov\u00fd syst\u00e9m na valid\u00e1ciu \u00fa\u010dinnosti skon\u0161truovan\u00e9ho transportn\u00e9ho syst\u00e9mu DARLINK. Modul\u00e1rna \u0161trukt\u00fara DARLINKu umo\u017en\u00ed za\u010datie novej l\u00ednie biomedic\u00ednsky orientovan\u00e9ho v\u00fdskumu so zna\u010dn\u00fdm dopadom na oblas\u0165 cielenej terapie. Konkr\u00e9tnymi cie\u013emi v\u00fdskumu v tejto t\u00e9me s\u00fa: i) v\u00fdvoj lipoprote\u00ednov\u00e9ho transportn\u00e9ho syst\u00e9mu lie\u010div, ii) v\u00fdskum optimaliz\u00e1cie komplexu DARPin\/lipoprote\u00ednov\u00fd transportn\u00fd syst\u00e9m lie\u010div, iii) anal\u00fdza z\u00e1chytu komplexu DARLINK\/lie\u010divo modelov\u00fdmi bunkov\u00fdmi syst\u00e9mami a iv) stanovenie mechanizmu protin\u00e1dorov\u00e9ho \u00fa\u010dinku komplexu DARLINK\/lie\u010divo.<\/p>\n\n\n\n

    Prij\u00edmaj\u00faci profesor:<\/strong> prof. RNDr. Pavol Mi\u0161kovsk\u00fd, DrSc., E-mail: pavol.miskovsky@upjs.sk<\/a><\/p>\n\n\n\n

    Zodpovedn\u00fd pracovn\u00edk:<\/strong> prof. RNDr. Pavol Mi\u0161kovsk\u00fd, DrSc.<\/p>\n\n\n\n

    Odborn\u00e9 po\u017eiadavky\/krit\u00e9ri\u00e1:<\/strong><\/p>\n\n\n\n

    Zisk titulu PhD. v \u0161tudijnom programe Biofyzika (resp. pr\u00edbuznom \u0161tudijnom programe, napr. Bioch\u00e9mia, Molekulov\u00e1 biol\u00f3gia), minim\u00e1lne tri publik\u00e1cie publikovan\u00e9 vo vedeck\u00fdch \u010dasopisoch veden\u00fdch v datab\u00e1ze WoS, minim\u00e1lne na jednej z nich prv\u00fd alebo kore\u0161ponduj\u00faci autor, sk\u00fasenosti s pr\u00e1cou v oblasti v\u00fdvoja transportn\u00fdch syst\u00e9mov lie\u010div a pr\u00e1cou s bunkov\u00fdmi kult\u00farami<\/p>\n\n\n\n

    3. – kotva2<\/a> N\u00e1zov t\u00e9my: Magnetoimpedan\u010dn\u00fd jav v multifunk\u010dn\u00fdch materi\u00e1loch.<\/strong><\/p>\n\n\n\n

    Anot\u00e1cia:<\/strong><\/p>\n\n\n\n

    Cie\u013eom t\u00e9my je presk\u00fama\u0165 mo\u017enos\u0165 vyu\u017eitia magnetoimpedan\u010dn\u00e9ho javu na sn\u00edmanie extern\u00fdch parametrov (teplota, tlak, pred\u013a\u017eenie a pod.) v multifunk\u010dn\u00fdch materi\u00e1loch (napr. magnetokalorick\u00fdch, s tvarovou pam\u00e4\u0165ou, a pod.) na b\u00e1ze amorfn\u00fdch resp. kry\u0161talick\u00fdch intermetalick\u00fdch zliatin. Vhodne zvolen\u00fdm tvarom a anizotropiou modifikova\u0165 odozvu magnetoimpedan\u010dn\u00e9ho javu na tieto extern\u00e9 parametre.<\/p>\n\n\n\n

    4.- kotva4<\/a> Prij\u00edmaj\u00faci profesor<\/strong><\/p>\n\n\n\n

    fffffffffffffffffffffffffffffffffffffffffffffffff<\/p>\n\n\n\n

    Detailnej\u0161ie poznanie mno\u017einovej \u0161trukt\u00fary re\u00e1lnych \u010d\u00edsel je d\u00f4le\u017eit\u00e9 napr\u00edklad pre lep\u0161ie pochopenie podstaty konvergencie, \u010do je v pozad\u00ed mnoh\u00fdch numerick\u00fdch itera\u010dn\u00fdch algoritmov. Na druhej strane, \u00fa\u010dinn\u00fdm n\u00e1strojom v\u00fdskumu \u0161trukt\u00fary re\u00e1lnych \u010d\u00edsel sa stala te\u00f3ria hier a te\u00f3ria miery.<\/p>\n\n\n\n

    Vedeck\u00e9 \u00falohy v r\u00e1mci tejto t\u00e9my s\u00fa zalo\u017een\u00e9 na s\u00fa\u010dasn\u00fdch poznatkoch v oblasti v\u00fdvoja transportn\u00fdch syst\u00e9mov pre cielen\u00fd transport lie\u010div. Navrhovan\u00fd transportn\u00fd syst\u00e9m lie\u010div (DARLINK) je zalo\u017een\u00fd na rekombinantnej inov\u00e1cii pozost\u00e1vaj\u00facej zo spojenia DARPinov \u2013 maj\u00facich vysok\u00fa afinitu pre v\u00e4zbu na \u0161pecifick\u00e9 receptory n\u00e1dorov\u00fdch buniek \u2013 a endog\u00e9nnych lipoprote\u00ednov\u00fdch nano\u010dast\u00edc, ktor\u00e9 maj\u00fa zna\u010dn\u00fa kapacitu pre naviazanie hydrof\u00f3bnych resp. amfifiln\u00fdch lie\u010div. Konkr\u00e9tne, navrhovan\u00fd transportn\u00fd syst\u00e9m bude skon\u0161truovan\u00fd f\u00faziou molek\u00fal LDL (alebo HDL) so \u0161pecifick\u00fdmi DARPinmi, ktor\u00e9 maj\u00fa vysok\u00fa afinitu pre naviazanie na HER2 receptory nach\u00e1dzaj\u00face sa na povrchu n\u00e1dorov\u00fdch buniek mlie\u010dnej \u017e\u013eazy a taktie\u017e DARPinmi vyselektovan\u00fdmi pre v\u00e4zbu na prirodzen\u00fa a mutovan\u00fa formu EGFR, ktor\u00e9 s\u00fa lokalizovan\u00e9 na povrchu buniek p\u013e\u00facnych n\u00e1dorov. Bunky n\u00e1doru mlie\u010dnej \u017e\u013eazy boli vybran\u00e9 ako modelov\u00fd syst\u00e9m na valid\u00e1ciu \u00fa\u010dinnosti skon\u0161truovan\u00e9ho transportn\u00e9ho syst\u00e9mu DARLINK. Modul\u00e1rna \u0161trukt\u00fara DARLINKu umo\u017en\u00ed za\u010datie novej l\u00ednie biomedic\u00ednsky orientovan\u00e9ho v\u00fdskumu so zna\u010dn\u00fdm dopadom na oblas\u0165 cielenej terapie. Konkr\u00e9tnymi cie\u013emi v\u00fdskumu v tejto t\u00e9me s\u00fa: i) v\u00fdvoj lipoprote\u00ednov\u00e9ho transportn\u00e9ho syst\u00e9mu lie\u010div, ii) v\u00fdskum optimaliz\u00e1cie komplexu DARPin\/lipoprote\u00ednov\u00fd transportn\u00fd syst\u00e9m lie\u010div, iii) anal\u00fdza z\u00e1chytu komplexu DARLINK\/lie\u010divo modelov\u00fdmi bunkov\u00fdmi syst\u00e9mami a iv) stanovenie mechanizmu protin\u00e1dorov\u00e9ho \u00fa\u010dinku komplexu DARLINK\/lie\u010divo.<\/p>\n\n\n\n

    <\/p>\n","protected":false},"excerpt":{"rendered":"

    Hlavn\u00fdm cie\u013eom tohto projektu je vyvin\u00fa\u0165 molekul\u00e1rnu met\u00f3du, pomocou ktorej budeme schopn\u00ed transformova\u0165 r\u00f4zne flavoprote\u00edny na \u00fa\u010dinn\u00e9 fotosenzibiliz\u00e1tory produkuj\u00face singletov\u00fd kysl\u00edk s vysok\u00fdm kvantov\u00fdm v\u00fd\u0165a\u017ekom. Vybran\u00e9 flavoprote\u00edny a vhodne navrhnut\u00e9 mutanty bud\u00fa exprimovan\u00e9 v Escherichia coli a purifikovan\u00e9 be\u017en\u00fdmi met\u00f3dami. Vytvoren\u00e9 prote\u00edny konjugujeme so \u0161pecifick\u00fdmi DARPin-mi (skupina navrhnut\u00fdch ankyr\u00ednov\u00fdch repetit\u00edvnych prote\u00ednov \u2013 prote\u00ednov\u00e1 skupina, ktor\u00e1 predstavuje anal\u00f3gy … Continued<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":13,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_editorskit_title_hidden":false,"_editorskit_reading_time":3,"_editorskit_is_block_options_detached":false,"_editorskit_block_options_position":"{}","footnotes":""},"class_list":["post-13900","page","type-page","status-publish","hentry"],"acf":{"acf_link_na_externy_obsah":""},"yoast_head":"\ntest kotva - testovacia<\/title>\n<meta name=\"robots\" content=\"noindex, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"test kotva - testovacia\" \/>\n<meta property=\"og:description\" content=\"Hlavn\u00fdm cie\u013eom tohto projektu je vyvin\u00fa\u0165 molekul\u00e1rnu met\u00f3du, pomocou ktorej budeme schopn\u00ed transformova\u0165 r\u00f4zne flavoprote\u00edny na \u00fa\u010dinn\u00e9 fotosenzibiliz\u00e1tory produkuj\u00face singletov\u00fd kysl\u00edk s vysok\u00fdm kvantov\u00fdm v\u00fd\u0165a\u017ekom. Vybran\u00e9 flavoprote\u00edny a vhodne navrhnut\u00e9 mutanty bud\u00fa exprimovan\u00e9 v Escherichia coli a purifikovan\u00e9 be\u017en\u00fdmi met\u00f3dami. 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