{"id":20475,"date":"2021-10-15T12:54:14","date_gmt":"2021-10-15T12:54:14","guid":{"rendered":"https:\/\/www.upjs.sk\/prirodovedecka-fakulta\/pracoviska\/ubev\/apu\/kmb\/studium\/dp\/"},"modified":"2022-11-13T21:32:24","modified_gmt":"2022-11-13T20:32:24","slug":"dp","status":"publish","type":"cpt_pracoviska","link":"https:\/\/www.upjs.sk\/prirodovedecka-fakulta\/pracoviska\/ustavy-pf\/ubev\/apu\/kmb\/studium\/dp\/","title":{"rendered":"Dizerta\u010dn\u00e9 pr\u00e1ce"},"content":{"rendered":"<h3>Preh\u013ead aktu\u00e1lne rie\u0161en\u00fdch dizerta\u010dn\u00fdch pr\u00e1c na na\u0161ej katedre<\/h3>\n<hr \/>\n<h4><strong>Biologick\u00e1 cena prenosnej rezistencie na\u00a0antibiotik\u00e1<\/strong><\/h4>\n<ul>\n<li><u>ved\u00faci<\/u>: doc. RNDr. Peter Prista\u0161, CSc. (peter.pristas@upjs.sk)<\/li>\n<li><u>anot\u00e1cia<\/u>: cie\u013eom pr\u00e1ce je identifikova\u0165 biologick\u00fa cenu prenosnej rezistencie na antibiotik\u00e1 u bakt\u00e9ri\u00ed a spozna\u0165 mechanizmy, ktor\u00fdmi s\u00fa mobiln\u00e9 g\u00e9nov\u00e9 elementy schopn\u00e9 t\u00fato cenu eliminova\u0165.<\/li>\n<\/ul>\n<h4 style=\"text-align:justify\"><strong>Fyziologick\u00e9 a\u00a0genetick\u00e9 mechanizmy adapt\u00e1cie mikrobi\u00e1lnych popul\u00e1ci\u00ed na\u00a0environment\u00e1lny stres<\/strong><\/h4>\n<ul>\n<li><u>ved\u00faci<\/u>: doc. RNDr. Peter Prista\u0161, CSc. (peter.pristas@upjs.sk);\u00a0konzultant:\u00a0RNDr. M\u00e1ria Piknov\u00e1, PhD.<\/li>\n<li><u>anot\u00e1cia<\/u>:\u00a0\u0160t\u00fadium extr\u00e9mnych prostred\u00ed v posledn\u00fdch rokoch viedlo k zauj\u00edmav\u00fdm objavom, ktor\u00e9 zmenili na\u0161e nazeranie na p\u00f4vod a vznik \u017eivota na tejto Zemi, ale umo\u017enilo aj biotechnologick\u00e9 vyu\u017eitie mikroorganizmov adaptovan\u00fdch na tieto prostredia. T\u00e9ma dizerta\u010dnej pr\u00e1ce je prim\u00e1rne zameran\u00e1 na \u0161t\u00fadium mikrobi\u00f3mu hypersalinn\u00fdch (slan\u00fdch) prame\u0148ov na Slovensku a izol\u00e1ciu, mikrobiologick\u00fa a genetick\u00fa charakteriz\u00e1ciu halofiln\u00fdch mikroorganizmov v t\u00fdchto prostrediach s cie\u013eom identifikova\u0165 nov\u00e9 druhy bakt\u00e9ri\u00ed, charakterizova\u0165 ich gen\u00f3m a fyziologick\u00e9 a molekulov\u00e9 adapt\u00e1cie na extr\u00e9mne prostredia. S cie\u013eom pochopenia molekulov\u00fdch adapt\u00e1ci\u00ed vybran\u00fdch extr\u00e9moz\u00fdmov na vysok\u00fa salinitu bud\u00fa tieto overexprimovan\u00e9 v heterologick\u00fdch hostite\u013eoch a bude sa \u0161tudova\u0165 molekulov\u00e1 dynamika a katalytick\u00e1 aktivita prote\u00ednov v z\u00e1vislosti na salinite prostredia.<\/li>\n<\/ul>\n<h4><strong>Fyziologick\u00e9 a\u00a0molekulov\u00e9 mechanizmy adapt\u00e1cie u\u00a0polyextr\u00e9motolerantn\u00fdch bakt\u00e9ri\u00ed<\/strong><\/h4>\n<ul>\n<li><u>ved\u00faci<\/u>: doc. RNDr. Peter Prista\u0161, CSc. (peter.pristas@upjs.sk)<\/li>\n<li><u>anot\u00e1cia<\/u>: cie\u013eom pr\u00e1ce je \u0161t\u00fadium je fyziologick\u00fdch a molekulov\u00fdch mechanizmov adapt\u00e1cie na environment\u00e1lny stres u bakt\u00e9ri\u00ed \u017eij\u00facich v prostrediach s minim\u00e1lne dvoma stresov\u00fdmi faktormi (pH, salinita, pr\u00edtomnos\u0165 \u0165a\u017ek\u00fdch kovov a antibiot\u00edk)<\/li>\n<\/ul>\n<h4><strong>Biodiverzita drevoznehodnocuj\u00facich h\u00fab ako zdroj nov\u00fdch biologicky akt\u00edvnych l\u00e1tok s\u00a0potenci\u00e1lnym vyu\u017eit\u00edm v\u00a0antimikrobi\u00e1lnej terapii<\/strong><\/h4>\n<ul>\n<li><u>ved\u00faci<\/u>: doc. RNDr. Peter Prista\u0161, CSc. (peter.pristas@upjs.sk);\u00a0konzultant:\u00a0RNDr. M\u00e1ria Piknov\u00e1, PhD.<\/li>\n<li><u>anot\u00e1cia<\/u>: neb\u00fdval\u00fd n\u00e1rast rezistencie vo\u010di antibiotik\u00e1m je v\u00e1\u017enym medic\u00ednskym probl\u00e9mom dne\u0161nej doby a ned\u00e1vne anal\u00fdzy nazna\u010duj\u00fa, \u017ee ochorenia vyvolan\u00e9 mikroorganizmami rezistentn\u00fdmi vo\u010di antibiotik\u00e1m sa v bl\u00edzkej dobe stan\u00fa hlavnou pr\u00ed\u010dinou \u00famrt\u00ed aj v rozvinut\u00fdch krajin\u00e1ch. Napriek pokrokom v syntetickej ch\u00e9mii sa pri antibakteri\u00e1lnej lie\u010dbe pou\u017e\u00edvaj\u00fa l\u00e1tky pr\u00edrodn\u00e9ho p\u00f4vodu. Aj ke\u010f prv\u00e9 \u0161iroko pou\u017e\u00edvan\u00e9 antibiotikum poch\u00e1dzalo pr\u00e1ve z h\u00fab, s rozvojom ch\u00e9mie sme v Eur\u00f3pe stratili inform\u00e1cie o mo\u017enom terapeutickom \u010di prevent\u00edvnom vyu\u017eit\u00ed h\u00fab a nimi produkovan\u00fdch biologicky akt\u00edvnych l\u00e1tok. Cie\u013eom pr\u00e1ce je vyu\u017eitie doteraz nepreb\u00e1danej biodiverzity drevoznehodnocuj\u00facich h\u00fab ako zdroja nov\u00fdch biologicky akt\u00edvnych l\u00e1tok s potenci\u00e1lnym vyu\u017eit\u00edm v antimikrobi\u00e1lnej terapii. V r\u00e1mci projektu budeme \u0161tudova\u0165 genetick\u00fa diverzitu vybran\u00fdch druhov drevoznehodnocuj\u00facich h\u00fab a met\u00f3dy pre ich in vitro kultiv\u00e1ciu; budeme analyzova\u0165 biologick\u00fa aktivitu extraktov t\u00fdchto h\u00fab a purifikova\u0165, zis\u0165ova\u0165 \u0161trukt\u00faru a antimikrobi\u00e1lnu aktivitu vybran\u00fdch l\u00e1tok produkovan\u00fdch hubami.<\/li>\n<\/ul>\n<h4><strong>Genetick\u00e1 diverzita a\u00a0genomika bakt\u00e9ri\u00ed v\u00a0biogeochemickom cykle s\u00edry<\/strong><\/h4>\n<ul>\n<li><u>ved\u00faci<\/u>: doc. RNDr. Peter Prista\u0161, CSc. (peter.pristas@upjs.sk);\u00a0konzultant:\u00a0RNDr. M\u00e1ria Piknov\u00e1, PhD.<\/li>\n<li><u>anot\u00e1cia<\/u>: bakt\u00e9rie sa vyskytuj\u00fa vo v\u0161etk\u00fdch pozemsk\u00fdch ekosyst\u00e9moch a \u010dasto pre\u017e\u00edvaj\u00fa aj v prostrediach, ktor\u00e9 s\u00fa z poh\u013eadu klasickej biol\u00f3gie absol\u00fatne nevhodn\u00e9 pre \u017eivot. S\u00fa s\u00fa\u010das\u0165ou bio-geochemick\u00fdch cyklov v\u0161etk\u00fdch prvkov a \u010dasto kr\u00e1t hlavnou hybnou silou mnoh\u00fdch pr\u00edrodn\u00fdch procesov. Bakt\u00e9rie podie\u013eaj\u00face sa na biogeochemickom cykle s\u00edry predstavuj\u00fa skupinu bakt\u00e9ri\u00ed s unik\u00e1tnymi sp\u00f4sobmi z\u00edskavania energie postaven\u00fdm na metabolizme s\u00edry. Tieto bakt\u00e9rie s\u00fa zauj\u00edmav\u00e9 \u010di u\u017e z h\u013eadiska z\u00e1kladn\u00e9ho poznania, ale aj v\u010faka environment\u00e1lnym dopadom ich metabolickej aktivity a mo\u017en\u00e9mu biotechnologick\u00e9mu vyu\u017eitiu. V na\u0161ich predch\u00e1dzaj\u00facich experimentoch sme dok\u00e1zali, \u017ee prostredia bohat\u00e9 na s\u00edru na Slovensku s\u00fa os\u00eddlen\u00e9 \u010dasto kr\u00e1t doteraz nezn\u00e1mymi druhmi bakt\u00e9ri\u00ed. Cie\u013eom pr\u00e1ce je s vyu\u017eit\u00edm kultiva\u010dn\u00fdch a nekultiva\u010dn\u00fdch postupov \u0161tudova\u0165 genetick\u00fa diverzitu\u00a0 bakt\u00e9ri\u00ed v biogeochemickom cykle s\u00edry, izolova\u0165 a charakterizova\u0165 nov\u00e9 druhy litoautotrofn\u00fdch s\u00edru oxiduj\u00facich bakt\u00e9ri\u00ed a s vyu\u017eit\u00edm genomick\u00fdch pr\u00edstupov \u0161tudova\u0165 evolu\u010dn\u00e9 vz\u0165ahy v tejto skupine bakt\u00e9ri\u00ed.<\/li>\n<\/ul>\n<h3>\u00a0<\/h3>\n<h3>Preh\u013ead vybran\u00fdch dizerta\u010dn\u00fdch pr\u00e1c z predo\u0161l\u00fdch rokov<\/h3>\n<hr \/>\n<ul>\n<li style=\"text-align: justify;\">Ekol\u00f3gia g\u00e9nov antibiotickej rezistencie<\/li>\n<li style=\"text-align: justify;\">Bakteri\u00e1lna mikrofl\u00f3ra tr\u00e1viaceho traktu v\u010dely medonosnej (<em>Apis mellifera<\/em>), so zameran\u00edm na\u00a0<em>Paenibacillus larvae<\/em>, p\u00f4vodcu moru v\u010delieho plodu<\/li>\n<\/ul>\n","protected":false},"author":1,"featured_media":0,"parent":20457,"menu_order":3,"template":"","meta":{"_acf_changed":false,"_editorskit_title_hidden":false,"_editorskit_reading_time":0,"_editorskit_is_block_options_detached":false,"_editorskit_block_options_position":"{}"},"categories":[],"tags":[],"tax_aktuality":[],"class_list":["post-20475","cpt_pracoviska","type-cpt_pracoviska","status-publish","hentry"],"acf":{"acf_link_na_externy_obsah":""},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Dizerta\u010dn\u00e9 pr\u00e1ce - Pr\u00edrodovedeck\u00e1 fakulta | UPJ\u0160<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.upjs.sk\/prirodovedecka-fakulta\/pracoviska\/ustavy-pf\/ubev\/apu\/kmb\/studium\/dp\/\" \/>\n<meta property=\"og:locale\" content=\"sk_SK\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Dizerta\u010dn\u00e9 pr\u00e1ce - Pr\u00edrodovedeck\u00e1 fakulta | UPJ\u0160\" \/>\n<meta property=\"og:description\" content=\"Preh\u013ead aktu\u00e1lne rie\u0161en\u00fdch dizerta\u010dn\u00fdch pr\u00e1c na na\u0161ej katedre Biologick\u00e1 cena prenosnej rezistencie na\u00a0antibiotik\u00e1 ved\u00faci: doc. 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