{"id":16484,"date":"2021-01-13T15:34:29","date_gmt":"2021-01-13T15:34:29","guid":{"rendered":"https:\/\/www.upjs.sk\/prirodovedecka-fakulta\/actual-news\/24146\/"},"modified":"2022-09-28T12:16:55","modified_gmt":"2022-09-28T12:16:55","slug":"24146","status":"publish","type":"cpt_aktualne_spravy","link":"https:\/\/www.upjs.sk\/prirodovedecka-fakulta\/actual-news\/24146\/","title":{"rendered":"Gen\u00f3m \u013eubovn\u00edka do posledn\u00e9ho p\u00edsmena"},"content":{"rendered":"<h3><strong>Prv\u00e9 genomick\u00e9 d\u00e1ta <em>Hypericum perforatum <\/em>a funk\u010dn\u00e1 valid\u00e1cia g\u00e9nu biosyntetickej dr\u00e1hy fytomelaton\u00ednu\u00a0<\/strong><\/h3>\n<p style=\"text-align: justify;\">Jedn\u00fdm z pr\u00edrodn\u00fdch zdrojov farmakodynamicky \u00fa\u010dinn\u00fdch l\u00e1tok, ktor\u00e9 patria dlhodobo k naj\u017eiadanej\u0161\u00edm\u00a0na svetovom trhu, je jedna z\u00a0najdlh\u0161ie pou\u017e\u00edvan\u00fdch lie\u010div\u00fdch rastl\u00edn <strong>\u013eubovn\u00edk bodkovan\u00fd <\/strong>&#8211; <em>Hypericum perforatum<\/em>. Napriek vysok\u00e9mu komer\u010dn\u00e9mu z\u00e1ujmu a evidentn\u00fdm bioakt\u00edvnym \u00fa\u010dinkom molekulov\u00e9 z\u00e1klady biosynt\u00e9zy profiluj\u00facich sekund\u00e1rnych metabolitov nie s\u00fa dostato\u010dne objasnen\u00e9. Detailn\u00e9mu \u0161t\u00fadiu br\u00e1nila aj absencia genomick\u00fdch d\u00e1t a aplik\u00e1cia pr\u00edstupov syntetickej biol\u00f3gie.<\/p>\n<p style=\"text-align: justify;\"><strong>Kolekt\u00edv <em>prof. RNDr. Evy \u010cell\u00e1rovej, DrSc<\/em>.<\/strong> z <strong>Katedry genetiky \u00dastavu<\/strong> <strong>biologick\u00fdch a ekologick\u00fdch vied Pr\u00edrodovedeckej fakulty Univerzity P. J. \u0160af\u00e1rika v Ko\u0161iciach<\/strong> spolu s t\u00edmom <em>prof. Wanga<\/em> <strong>z Univerzity v meste Xi\u00b4an v \u010c\u00edne<\/strong> a v spolupr\u00e1ci s <strong>Univerzitou v Ottawe publikovali prv\u00fdkr\u00e1t genomick\u00e9 d\u00e1ta<\/strong> tohto v\u00fdznamn\u00e9ho producenta farmakodynamicky \u00fa\u010dinn\u00fdch l\u00e1tok. Spomedzi 40 kandid\u00e1tnych g\u00e9nov biosynt\u00e9zy profiluj\u00facich sekund\u00e1rnych metabolitov hyperic\u00ednu, hyperfor\u00ednu a melaton\u00ednu prin\u00e1\u0161a pr\u00e1ca v\u00fdsledky experiment\u00e1lnej funk\u010dnej valid\u00e1cie g\u00e9nu k\u00f3duj\u00faceho enz\u00fdm N-acetylseroton\u00edn-O-metyltransfer\u00e1za (ASMT), ktor\u00fd je posledn\u00fdm enz\u00fdmom v biosynt\u00e9ze melaton\u00ednu, a jeho subcelul\u00e1rnu lokaliz\u00e1ciu.<\/p>\n<p style=\"text-align: justify;\">Z uveden\u00e9ho d\u00f4vodu <strong>bola t\u00e1to pr\u00e1ca publikovan\u00e1 v renomovanom endokrinologickom \u010dasopise <em>Journal of Pineal Research <\/em>(<a href=\"https:\/\/doi.org\/10.1111\/jpi.12709\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.1111\/jpi.12709<\/a>, IF>15)<\/strong>. Tento horm\u00f3n, ktor\u00fd bol p\u00f4vodne identifikovan\u00fd v \u017eivo\u010d\u00edchoch a v \u013eudskom organizme a nesk\u00f4r aj v niektor\u00fdch rastlin\u00e1ch, je pr\u00edkladom ortol\u00f3gneho charakteru biosyntetick\u00fdch g\u00e9nov a evolu\u010dnej konzervat\u00edvnosti. Jeho funkcia v rastlin\u00e1ch je predov\u0161etk\u00fdm v odpovedi na stresov\u00e9 podmienky, \u010do potvrdili aj v\u00fdsledky tejto pr\u00e1ce.<\/p>\n<p style=\"text-align: justify;\">Gen\u00f3m <em>H. perforatum <\/em>m\u00e1 ve\u013ekos\u0165 takmer 400 Mb s pribli\u017ene 50% zast\u00fapen\u00edm repetit\u00edvnych sekvenci\u00ed, z ktor\u00fdch takmer polovicu tvoria transpoz\u00f3ny. Je zauj\u00edmav\u00e9, \u017ee v gen\u00f3me <em>H. perforatum <\/em>s\u00fa pr\u00edtomn\u00e9 v\u0161etky kateg\u00f3rie transpoz\u00f3nov, ktor\u00e9 pozn\u00e1me aj z \u013eudsk\u00e9ho gen\u00f3mu &#8211; DNA transpoz\u00f3ny, LINE, SINE (<em>long\/short interspersed nuclear elements<\/em>), LTR <em>(long terminal repeats) <\/em>s prevl\u00e1daj\u00facim zast\u00fapen\u00edm LTR. Na z\u00e1klade predikci\u00ed obsahuje gen\u00f3m <em>H. perforatum <\/em>asi 29 000 g\u00e9nov, z nich asi 20 000 je v s\u00falade s na\u0161imi predch\u00e1dzaj\u00facimi predikciami z transkriptomick\u00fdch \u0161t\u00fadi\u00ed. Komparat\u00edvne \u0161t\u00fadium s genomick\u00fdmi d\u00e1tami modelov\u00fdch rastl\u00edn odhalilo pr\u00edtomnos\u0165 asi 1000 g\u00e9nov\u00fdch rod\u00edn \u0161pecifick\u00fdch pre <em>H. perforatum, <\/em>ktor\u00e9 pravdepodobne obsahuj\u00fa aj mno\u017einu g\u00e9nov, v ktor\u00fdch sa nach\u00e1dzaj\u00fa biosyntetick\u00e9 g\u00e9ny hyperic\u00ednu, ke\u010f\u017ee tento bioakt\u00edvny metabolit je v rastlinnej r\u00ed\u0161i pr\u00edtomn\u00fd len v niektor\u00fdch z\u00e1stupcoch rodu <em>Hypericum<\/em>.<\/p>\n<p style=\"text-align: justify;\">Po predch\u00e1dzaj\u00facich transkriptomick\u00fdch pr\u00e1cach kolekt\u00edvu a pilotnej skr\u00edningovej \u0161t\u00fadii <em>in silico <\/em>o miRNAs a ich cie\u013eov\u00fdch sekvenci\u00e1ch v rode <em>Hypericum <\/em><strong>predstavuj\u00fa<\/strong> <strong>genomick\u00e9 d\u00e1ta nov\u00fd zdroj pre \u0161t\u00fadium biosyntetick\u00fdch g\u00e9nov v\u00fdznamn\u00fdch sekund\u00e1rnych metabolitov a ich genetickej a epigenetickej regul\u00e1cie s perspekt\u00edvou biotechnologickej produkcie v homol\u00f3gnych alebo heterol\u00f3gnych syst\u00e9moch.<\/strong><\/p>\n<p style=\"text-align: center;\"><strong><a href=\"\/public\/media\/24146\/obr_1.jpg\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" alt=\"\" src=\"http:\/\/www.upjs.sk\/\/app\/uploads\/sites\/10\/2022\/09\/obr_1.jpg\" style=\"width:50%\" \/><\/a><\/strong><\/p>\n<p style=\"text-align: center;\"><strong><em>Obr. 1<\/em><\/strong><em>\u00a0\u00a0 Sch\u00e9ma biosynt\u00e9zy melaton\u00ednu <\/em><\/p>\n<p style=\"text-align: center;\"><a href=\"\/public\/media\/24146\/obr_2.jpg\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" alt=\"\" src=\"http:\/\/www.upjs.sk\/\/app\/uploads\/sites\/10\/2022\/09\/obr_2.jpg\" style=\"width:50%\" \/><\/a><\/p>\n<p style=\"text-align: center;\"><strong><em>Obr. 2<\/em><\/strong><em>\u00a0 \u00a0Subcelul\u00e1rna lokaliz\u00e1cia enz\u00fdmu ASMT<\/em><em> <\/em><\/p>\n<p><em>Wen Zhou, Ying Wang, Bin Li, Linda Petijov\u00e1, Suying Hu, Qian Zhang, Junfeng NiuDonghao Wang, Shiqiang Wang, Yang Dong, Eva \u010cell\u00e1rov\u00e1, Zhezhi Wang<\/em> (2020):<br \/>\n<strong>Whole\u2010genome sequence data of Hypericum perforatum and functional characterization of melatonin biosynthesis by N\u2010acetylserotonin O\u2010methyltransferase.<\/strong><br \/>\n<em>Journal of Pineal Re<\/em>search, 00:e1270<br \/>\n<a href=\"https:\/\/doi.org\/10.1111\/jpi.12709\">https:\/\/doi.org\/10.1111\/jpi.12709<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p style=\"text-align: justify;\">Napriek pokrokom v\u00a0syntetickej ch\u00e9mii s\u00fa pr\u00edroda a\u00a0lie\u010div\u00e9 rastliny st\u00e1le bohat\u00fdm zdrojom l\u00e1tok pou\u017e\u00edvan\u00fdch v\u00a0prevencii a\u00a0lie\u010dbe mnoh\u00fdch ochoren\u00ed. T\u00edm katedry genetiky \u00daBEV PF UPJ\u0160 <span style=\"color:#A52A2A\"><strong>pod veden\u00edm profesorky Evy \u010cell\u00e1rovej<\/strong><\/span> sa v\u00a0spolupr\u00e1ci s\u00a0v\u00fdskumn\u00edkmi z \u010c\u00edny a Kanady podie\u013eal na charakteriz\u00e1cii kompletn\u00e9ho gen\u00f3mu jednej z\u00a0najdlh\u0161ie pou\u017e\u00edvan\u00fdch lie\u010div\u00fdch rastl\u00edn, \u013eubovn\u00edka bodkovan\u00e9ho (<em>Hypericum perforatum<\/em>) a\u00a0na <span style=\"color:#A52A2A\"><strong>objasnen\u00ed biosyntetickej dr\u00e1hy melaton\u00ednu<\/strong>, ktor\u00fd bol publikovan\u00fd <strong>v\u00a0prest\u00ed\u017enom \u010dasopise Journal of Pineal Research<\/strong><\/span><\/p>\n","protected":false},"author":1,"featured_media":16486,"menu_order":0,"template":"","meta":{"_editorskit_title_hidden":false,"_editorskit_reading_time":0,"_editorskit_is_block_options_detached":false,"_editorskit_block_options_position":"{}"},"categories":[],"tags":[],"tax_aktuality":[],"class_list":["post-16484","cpt_aktualne_spravy","type-cpt_aktualne_spravy","status-publish","has-post-thumbnail","hentry"],"acf":{"acf_link_na_externy_obsah":""},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Gen\u00f3m \u013eubovn\u00edka do posledn\u00e9ho p\u00edsmena - Pr\u00edrodovedeck\u00e1 fakulta | UPJ\u0160<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.upjs.sk\/prirodovedecka-fakulta\/actual-news\/24146\/\" \/>\n<meta property=\"og:locale\" content=\"sk_SK\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Gen\u00f3m \u013eubovn\u00edka do posledn\u00e9ho p\u00edsmena - Pr\u00edrodovedeck\u00e1 fakulta | UPJ\u0160\" \/>\n<meta property=\"og:description\" content=\"Napriek pokrokom v\u00a0syntetickej ch\u00e9mii s\u00fa pr\u00edroda a\u00a0lie\u010div\u00e9 rastliny st\u00e1le bohat\u00fdm zdrojom l\u00e1tok pou\u017e\u00edvan\u00fdch v\u00a0prevencii a\u00a0lie\u010dbe mnoh\u00fdch ochoren\u00ed. T\u00edm katedry genetiky \u00daBEV PF UPJ\u0160 pod veden\u00edm profesorky Evy \u010cell\u00e1rovej sa v\u00a0spolupr\u00e1ci s\u00a0v\u00fdskumn\u00edkmi z \u010c\u00edny a Kanady podie\u013eal na charakteriz\u00e1cii kompletn\u00e9ho gen\u00f3mu jednej z\u00a0najdlh\u0161ie pou\u017e\u00edvan\u00fdch lie\u010div\u00fdch rastl\u00edn, \u013eubovn\u00edka bodkovan\u00e9ho (Hypericum perforatum) a\u00a0na objasnen\u00ed biosyntetickej dr\u00e1hy melaton\u00ednu, ktor\u00fd bol publikovan\u00fd v\u00a0prest\u00ed\u017enom \u010dasopise Journal of Pineal Research\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.upjs.sk\/prirodovedecka-fakulta\/actual-news\/24146\/\" \/>\n<meta property=\"og:site_name\" content=\"Pr\u00edrodovedeck\u00e1 fakulta | UPJ\u0160\" \/>\n<meta property=\"article:modified_time\" content=\"2022-09-28T12:16:55+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.upjs.sk\/app\/uploads\/sites\/10\/2022\/09\/obr_2.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"599\" \/>\n\t<meta property=\"og:image:height\" content=\"899\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Predpokladan\u00fd \u010das \u010d\u00edtania\" \/>\n\t<meta name=\"twitter:data1\" content=\"3 min\u00faty\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.upjs.sk\/prirodovedecka-fakulta\/actual-news\/24146\/\",\"url\":\"https:\/\/www.upjs.sk\/prirodovedecka-fakulta\/actual-news\/24146\/\",\"name\":\"Gen\u00f3m \u013eubovn\u00edka do posledn\u00e9ho p\u00edsmena - 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